naturallypixie (![[personal profile]](https://www.dreamwidth.org/img/silk/identity/user.png)
naturallypixie) wrote2005-03-13 07:30 pm
naturallypixie) wrote2005-03-13 07:30 pm
What to do, what to do
It has been a while since I wrote anything worthwhile here and I am afraid that today will be no exception. I have been spending most of my time doing nothing at work. I was given several different projects, all of which went nowhere after I immediately jumped on doing the research for. But, I have finally gotten a project and actual DNA to do the work on. If you are scientifically minded, read on...if not, feel free to skip this next bit, I'll let you know when it is okay to read again.
I am doing two projects. The first is a methylation analysis of the p57 gene promoter in different types of thyroid tumors. This could be a very exciting methylation versus chromatin configuration experiment. The second project is much more long term. I will be constructing differential ChIP libraries from a prostate cell line, and if it works, I'll be doing more libraries from other cell types. The purpose of these libraries is to see what genes are differentially methylated after the addition of a stimulus. In the case of the prostate cell line, the stimulus will be 5-dihydroxy testosterone (DHT) which binds to the androgen receptor and is then translocated to the nucleus and those two molecules along with some other stuff, directly binds the DNA. I'll be doing two rounds of ChIP with anti-AR antibody.
EVERYBODY ELSE CAN READ AGAIN
Yesterday I wanted to go to a meeting of English speaking women here in Naples, but alas, I could not find the place. I received very sketchy directions from a random lady in the market down the street...They meet behind the Anglican church near Piazza Amadeo at 10 the second Saturday of every month. The really sad thing is that I brought a new friend along, she has been here a little longer than we have, and we totally went on a wild goose chase! I felt very bad about this. In fact, I was disappointed on several levels. *sigh* I will try again next month. It would be really great if the group's website goes back up between now and then so that I can get an address.
Finally, there is a new smell in our apartment, and neither one of us can figure out it's origin. Bart can smell it. That says something.
I am doing two projects. The first is a methylation analysis of the p57 gene promoter in different types of thyroid tumors. This could be a very exciting methylation versus chromatin configuration experiment. The second project is much more long term. I will be constructing differential ChIP libraries from a prostate cell line, and if it works, I'll be doing more libraries from other cell types. The purpose of these libraries is to see what genes are differentially methylated after the addition of a stimulus. In the case of the prostate cell line, the stimulus will be 5-dihydroxy testosterone (DHT) which binds to the androgen receptor and is then translocated to the nucleus and those two molecules along with some other stuff, directly binds the DNA. I'll be doing two rounds of ChIP with anti-AR antibody.
EVERYBODY ELSE CAN READ AGAIN
Yesterday I wanted to go to a meeting of English speaking women here in Naples, but alas, I could not find the place. I received very sketchy directions from a random lady in the market down the street...They meet behind the Anglican church near Piazza Amadeo at 10 the second Saturday of every month. The really sad thing is that I brought a new friend along, she has been here a little longer than we have, and we totally went on a wild goose chase! I felt very bad about this. In fact, I was disappointed on several levels. *sigh* I will try again next month. It would be really great if the group's website goes back up between now and then so that I can get an address.
Finally, there is a new smell in our apartment, and neither one of us can figure out it's origin. Bart can smell it. That says something.